diff --git a/wp-admin/includes/class-walker-nav-menu-checklist.php b/wp-admin/includes/class-walker-nav-menu-checklist.php
index 81c088420c..6cacf1874a 100644
--- a/wp-admin/includes/class-walker-nav-menu-checklist.php
+++ b/wp-admin/includes/class-walker-nav-menu-checklist.php
@@ -62,7 +62,8 @@ class Walker_Nav_Menu_Checklist extends Walker_Nav_Menu {
 	 *
 	 * @since 3.0.0
 	 *
-	 * @global int $_nav_menu_placeholder
+	 * @global int        $_nav_menu_placeholder
+	 * @global int|string $nav_menu_selected_id
 	 *
 	 * @param string $output Used to append additional content (passed by reference).
 	 * @param object $item   Menu item data object.
@@ -71,7 +72,7 @@ class Walker_Nav_Menu_Checklist extends Walker_Nav_Menu {
 	 * @param int    $id     Not used.
 	 */
 	public function start_el( &$output, $item, $depth = 0, $args = array(), $id = 0 ) {
-		global $_nav_menu_placeholder;
+		global $_nav_menu_placeholder, $nav_menu_selected_id;
 
 		$_nav_menu_placeholder = ( 0 > $_nav_menu_placeholder ) ? intval( $_nav_menu_placeholder ) - 1 : -1;
 		$possible_object_id    = isset( $item->post_type ) && 'nav_menu_item' == $item->post_type ? $item->object_id : $_nav_menu_placeholder;
@@ -81,7 +82,7 @@ class Walker_Nav_Menu_Checklist extends Walker_Nav_Menu {
 
 		$output .= $indent . '<li>';
 		$output .= '<label class="menu-item-title">';
-		$output .= '<input type="checkbox" class="menu-item-checkbox';
+		$output .= '<input type="checkbox"' . wp_nav_menu_disabled_check( $nav_menu_selected_id, false ) . ' class="menu-item-checkbox';
 
 		if ( ! empty( $item->front_or_home ) ) {
 			$output .= ' add-to-top';
diff --git a/wp-admin/includes/nav-menu.php b/wp-admin/includes/nav-menu.php
index cb0bbe6449..5a109f9b3e 100644
--- a/wp-admin/includes/nav-menu.php
+++ b/wp-admin/includes/nav-menu.php
@@ -255,23 +255,25 @@ function wp_nav_menu_taxonomy_meta_boxes() {
 }
 
 /**
- * Check whether to disable the Menu Locations meta box submit button
+ * Check whether to disable the Menu Locations meta box submit button and inputs.
  *
  * @since 3.6.0
+ * @since 5.3.1 The `$echo` parameter was added.
  *
  * @global bool $one_theme_location_no_menus to determine if no menus exist
  *
- * @param int|string $nav_menu_selected_id (id, name or slug) of the currently-selected menu
- * @return string Disabled attribute if at least one menu exists, false if not
+ * @param int|string $nav_menu_selected_id ID, name, or slug of the currently selected menu.
+ * @param bool       $echo                 Whether to echo or just return the string.
+ * @return string|false Disabled attribute if at least one menu exists, false if not.
  */
-function wp_nav_menu_disabled_check( $nav_menu_selected_id ) {
+function wp_nav_menu_disabled_check( $nav_menu_selected_id, $echo = true ) {
 	global $one_theme_location_no_menus;
 
 	if ( $one_theme_location_no_menus ) {
 		return false;
 	}
 
-	return disabled( $nav_menu_selected_id, 0 );
+	return disabled( $nav_menu_selected_id, 0, $echo );
 }
 
 /**
@@ -292,12 +294,12 @@ function wp_nav_menu_item_link_meta_box() {
 		<input type="hidden" value="custom" name="menu-item[<?php echo $_nav_menu_placeholder; ?>][menu-item-type]" />
 		<p id="menu-item-url-wrap" class="wp-clearfix">
 			<label class="howto" for="custom-menu-item-url"><?php _e( 'URL' ); ?></label>
-			<input id="custom-menu-item-url" name="menu-item[<?php echo $_nav_menu_placeholder; ?>][menu-item-url]" type="text" class="code menu-item-textbox" placeholder="https://" />
+			<input id="custom-menu-item-url" name="menu-item[<?php echo $_nav_menu_placeholder; ?>][menu-item-url]" type="text"<?php wp_nav_menu_disabled_check( $nav_menu_selected_id ); ?> class="code menu-item-textbox" placeholder="https://" />
 		</p>
 
 		<p id="menu-item-name-wrap" class="wp-clearfix">
 			<label class="howto" for="custom-menu-item-name"><?php _e( 'Link Text' ); ?></label>
-			<input id="custom-menu-item-name" name="menu-item[<?php echo $_nav_menu_placeholder; ?>][menu-item-title]" type="text" class="regular-text menu-item-textbox" />
+			<input id="custom-menu-item-name" name="menu-item[<?php echo $_nav_menu_placeholder; ?>][menu-item-title]" type="text"<?php wp_nav_menu_disabled_check( $nav_menu_selected_id ); ?> class="regular-text menu-item-textbox" />
 		</p>
 
 		<p class="button-controls wp-clearfix">
@@ -569,7 +571,7 @@ function wp_nav_menu_item_post_type_meta_box( $object, $box ) {
 			?>
 			<p class="quick-search-wrap">
 				<label for="quick-search-posttype-<?php echo $post_type_name; ?>" class="screen-reader-text"><?php _e( 'Search' ); ?></label>
-				<input type="search" class="quick-search" value="<?php echo $searched; ?>" name="quick-search-posttype-<?php echo $post_type_name; ?>" id="quick-search-posttype-<?php echo $post_type_name; ?>" />
+				<input type="search"<?php wp_nav_menu_disabled_check( $nav_menu_selected_id ); ?> class="quick-search" value="<?php echo $searched; ?>" name="quick-search-posttype-<?php echo $post_type_name; ?>" id="quick-search-posttype-<?php echo $post_type_name; ?>" />
 				<span class="spinner"></span>
 				<?php submit_button( __( 'Search' ), 'small quick-search-submit hide-if-js', 'submit', false, array( 'id' => 'submit-quick-search-posttype-' . $post_type_name ) ); ?>
 			</p>
@@ -651,7 +653,7 @@ function wp_nav_menu_item_post_type_meta_box( $object, $box ) {
 
 		<p class="button-controls wp-clearfix" data-items-type="posttype-<?php echo esc_attr( $post_type_name ); ?>">
 			<span class="list-controls hide-if-no-js">
-				<input type="checkbox" id="<?php echo esc_attr( $post_type_name . '-tab' ); ?>" class="select-all" />
+				<input type="checkbox"<?php wp_nav_menu_disabled_check( $nav_menu_selected_id ); ?> id="<?php echo esc_attr( $post_type_name . '-tab' ); ?>" class="select-all" />
 				<label for="<?php echo esc_attr( $post_type_name . '-tab' ); ?>"><?php _e( 'Select All' ); ?></label>
 			</span>
 
@@ -880,7 +882,7 @@ function wp_nav_menu_item_taxonomy_meta_box( $object, $box ) {
 
 		<p class="button-controls wp-clearfix" data-items-type="taxonomy-<?php echo esc_attr( $taxonomy_name ); ?>">
 			<span class="list-controls hide-if-no-js">
-				<input type="checkbox" id="<?php echo esc_attr( $taxonomy_name . '-tab' ); ?>" class="select-all" />
+				<input type="checkbox"<?php wp_nav_menu_disabled_check( $nav_menu_selected_id ); ?> id="<?php echo esc_attr( $taxonomy_name . '-tab' ); ?>" class="select-all" />
 				<label for="<?php echo esc_attr( $taxonomy_name . '-tab' ); ?>"><?php _e( 'Select All' ); ?></label>
 			</span>
 
diff --git a/wp-includes/version.php b/wp-includes/version.php
index da7b1912ec..d0c5c12ffc 100644
--- a/wp-includes/version.php
+++ b/wp-includes/version.php
@@ -13,7 +13,7 @@
  *
  * @global string $wp_version
  */
-$wp_version = '5.3.1-alpha-46841';
+$wp_version = '5.3.1-alpha-46843';
 
 /**
  * Holds the WordPress DB revision, increments when changes are made to the WordPress DB schema.