diff --git a/esphome/components/i2s_audio/microphone/__init__.py b/esphome/components/i2s_audio/microphone/__init__.py
index 48d4d28f8e..089e796ae0 100644
--- a/esphome/components/i2s_audio/microphone/__init__.py
+++ b/esphome/components/i2s_audio/microphone/__init__.py
@@ -2,7 +2,7 @@ import esphome.config_validation as cv
 import esphome.codegen as cg
 
 from esphome import pins
-from esphome.const import CONF_ID, CONF_NUMBER
+from esphome.const import CONF_CHANNEL, CONF_ID, CONF_NUMBER
 from esphome.components import microphone, esp32
 from esphome.components.adc import ESP32_VARIANT_ADC1_PIN_TO_CHANNEL, validate_adc_pin
 
@@ -25,6 +25,12 @@ I2SAudioMicrophone = i2s_audio_ns.class_(
     "I2SAudioMicrophone", I2SAudioIn, microphone.Microphone, cg.Component
 )
 
+i2s_channel_fmt_t = cg.global_ns.enum("i2s_channel_fmt_t")
+CHANNELS = {
+    "left": i2s_channel_fmt_t.I2S_CHANNEL_FMT_ONLY_LEFT,
+    "right": i2s_channel_fmt_t.I2S_CHANNEL_FMT_ONLY_RIGHT,
+}
+
 INTERNAL_ADC_VARIANTS = [esp32.const.VARIANT_ESP32]
 PDM_VARIANTS = [esp32.const.VARIANT_ESP32, esp32.const.VARIANT_ESP32S3]
 
@@ -47,6 +53,7 @@ BASE_SCHEMA = microphone.MICROPHONE_SCHEMA.extend(
     {
         cv.GenerateID(): cv.declare_id(I2SAudioMicrophone),
         cv.GenerateID(CONF_I2S_AUDIO_ID): cv.use_id(I2SAudioComponent),
+        cv.Optional(CONF_CHANNEL, default="right"): cv.enum(CHANNELS),
     }
 ).extend(cv.COMPONENT_SCHEMA)
 
@@ -86,4 +93,6 @@ async def to_code(config):
         cg.add(var.set_din_pin(config[CONF_I2S_DIN_PIN]))
         cg.add(var.set_pdm(config[CONF_PDM]))
 
+    cg.add(var.set_channel(CHANNELS[config[CONF_CHANNEL]]))
+
     await microphone.register_microphone(var, config)
diff --git a/esphome/components/i2s_audio/microphone/i2s_audio_microphone.cpp b/esphome/components/i2s_audio/microphone/i2s_audio_microphone.cpp
index 0f45cf95c6..9452762e94 100644
--- a/esphome/components/i2s_audio/microphone/i2s_audio_microphone.cpp
+++ b/esphome/components/i2s_audio/microphone/i2s_audio_microphone.cpp
@@ -49,7 +49,7 @@ void I2SAudioMicrophone::start_() {
       .mode = (i2s_mode_t) (I2S_MODE_MASTER | I2S_MODE_RX),
       .sample_rate = 16000,
       .bits_per_sample = I2S_BITS_PER_SAMPLE_16BIT,
-      .channel_format = I2S_CHANNEL_FMT_ONLY_RIGHT,
+      .channel_format = this->channel_,
       .communication_format = I2S_COMM_FORMAT_STAND_I2S,
       .intr_alloc_flags = ESP_INTR_FLAG_LEVEL1,
       .dma_buf_count = 4,
diff --git a/esphome/components/i2s_audio/microphone/i2s_audio_microphone.h b/esphome/components/i2s_audio/microphone/i2s_audio_microphone.h
index e704ed2915..acc7d2b45a 100644
--- a/esphome/components/i2s_audio/microphone/i2s_audio_microphone.h
+++ b/esphome/components/i2s_audio/microphone/i2s_audio_microphone.h
@@ -28,6 +28,8 @@ class I2SAudioMicrophone : public I2SAudioIn, public microphone::Microphone, pub
   }
 #endif
 
+  void set_channel(i2s_channel_fmt_t channel) { this->channel_ = channel; }
+
  protected:
   void start_();
   void stop_();
@@ -40,6 +42,7 @@ class I2SAudioMicrophone : public I2SAudioIn, public microphone::Microphone, pub
 #endif
   bool pdm_{false};
   std::vector<uint8_t> buffer_;
+  i2s_channel_fmt_t channel_;
 
   HighFrequencyLoopRequester high_freq_;
 };